Science Homework Help. University of Toronto Molecular Methods in Mutagenesis Questions
Please answer the questions below in full sentences- I will tip for a great answer, thanks!
1. Your graduate student has created the
following DNA sequence containing a cyclobutane pyrimidine dimer (denoted by
**), as well as several primers to use for primer extension. This will be used
as a test substrate to establish whether Pol4202 can bypass the lesion. Describe
the necessary biochemical components that must be added to carry out this
experiment and why each is required.
2. Interpret the results of the gel below (which was performed using the template and primers from Part A, above, with and without dimers as indicated at the top of each lane). Does Pol4202 possess the hypothesized activity? Recall that Polδ is a replicative polymerase. Describe the expected outcome on this gel for both successful and unsuccessful lesion bypass.
